Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae.
نویسندگان
چکیده
Antiserum to a purified type R lipopolysaccharide antigen isolated from Neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" N. gonorrhoeae. Cultures of Corynebacterium vaginalis, N. meningitidis, N. catarrhalis, N. sicca, and N. lactamicus were also tested in the slide agglutination procedure. The addition of both deoxyribonuclease and ribonuclease (1 mg/ml) to the cell suspension medium of phosphate-buffered saline improved the sensitivity and specificity of the agglutination reaction for N. gonorrhoeae. Problems relating to the agglutination test as an aid in identification of N. gonorrhoeae are discussed.
منابع مشابه
Identification of Neisseria gonorrhoeae from primary cultures by a slide agglutination test.
Hen antigonococcal lipopolysaccharide hen serum was used in a simple slide agglutination test for the identification of Neisseria gonorrhoeae from primary isolates.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 9 4 شماره
صفحات -
تاریخ انتشار 1979